Anti-PAGR1 (PA1)

产品说明

一般描述

PAXIP1-associated glutamate-rich protein 1 (UniProt: Q9BTK6; also known as Glutamate-rich co-activator interacting with SRC1, GAS, PAXIP1-associated protein 1, PTIP-associated protein 1) is encoded by the PAGR1 (also known as C16orf53, PA1) gene (Gene ID: 79447) in human. It associates with the histone methyltransferase MLL3/4 complex, which suggests that it plays a role in epigenetic and transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL3/4 complex. It is proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL3/4 complex. During immunoglobulin class switching in activated B-cells, it is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL3/4 complex. PAGR1 protein is reported to act as a transcriptional cofactor for estrogen receptor. It interacts with GR (NR3C1) and inhibits the GR transactivation.

特异性

This polyclonal antibody detects PAXIP1-associated glutamate-rich protein 1 in human and mouse.

免疫原

His-tagged full-length human recombinant PAXIP1-associated protein 1.

应用

Anti-PAGR1 (PA1), Cat. No. ABE1863, is a highly specific rabbit polyclonal antibody that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A representative lot detected PAGR1 (PA1) in Western Blotting applications (Lee, J.E., et. al. (2013). Elife. 2:e01503; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEF′s (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

质量

Evaluated by Western Blotting in HEK293 cell nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract.

目标描述

~42 kDa observed; 27.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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