产品说明
一般描述
ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.
Anti-ARF6 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ARFAG produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. class III includes only human Arf6. The ADP-ribosylation factors (Arf) family of proteins, which belong to the Ras (rat sarcoma viral oncogene homolog) family, are low molecular weight proteins capable of binding GTP.
应用
Anti-ARF6 antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.
生化/生理作用
ADP-ribosylation factor 6 (Arf6) is present at the plasma membrane and on endosomal membranes where it regulates the flow of trafficking into and out of the cell, and regulates the actin cytoskeleton on the plasma membrane. ARF6 activates phospholipase D (PLD) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K) to generate phosphatidic acid and PIP2 (phosphotidylinositol 4,5-bisphosphate), respectively.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705572 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | ARFAG, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 18 kDa |
| species reactivity | canine, rat, bovine, human |
| 浓度 | 2 mg/mL |
| technique(s) | western blot: 1-2 μg/mL using MDCK total cell extract. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P62330 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ARF6(382) rat ... Arf6(79121) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
