产品说明
一般描述
Monoclonal Anti- EPS8 (mouse IgG2a isotype) is derived from the hybridoma EPS8-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8 (GeneID: 2059), conjugated to Key loop hemocyanin (KLH). Epidermal growth factor receptor kinase substrate 8 (EPS8) is also known as DFNB102 (autosomal recessive deafness-102). EPS8 is also found to be an actin filament barbed end capping protein.
免疫原
synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8
应用
Monoclonal Anti-EPS8 antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
- flow cytometry
生化/生理作用
Epidermal growth factor receptor kinase substrate 8 (EPS8) serves as a substrate for the kinase activity of epidermal growth factor receptor (EGFR). It is essential for cell motility and regulation of actin filaments growth. EPS8 scaffolds together with Abl interactor 1 (Abi1) and SOS Ras/Rac guanine nucleotide exchange factor 1 (Sos1) to a trimeric complex which is required for activation of the small guanosine triphosphatase (GTPase) Rac. On the other hand, EPS8 form complexes with related to the N terminus of tre oncogene (RN-tre), a Rab5 GTPase activating protein, to inhibit EGFR internalization. EPS8 is involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration, cancer cell migration and invasion. It is reported to be expressed at elevated levels in a range of human malignancies. This protein is also involved in spine morphogenesis and plasticity and may characterize forms of intellectual disabilities. For instance, reduced EPS8 levels were reported in brains of autism patients.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | EPS8-2 |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~90 kDa |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 10-20 μg/test using using A549 cells. immunoblotting: 2.5-5 μg/mL using using whole extracts of HepG2 cells. immunofluorescence: 10-20 μg/mL using using whole extracts of A549 cells. immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | Q12929 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... EPS8(2059) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
