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Lambda Phage DNA, Methylated from Escherichia coli host strain W3110

品牌
Sigma-Aldrich
CAS
91080-14-7
货号
D9768
规格纯度
lyophilized powder
参考价格
702.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This methylated lambda DNA is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I.

特异性

Unique restriction sites: Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I. Methylated lambda is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I.

应用

Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used for immunoprecipitation in ChIP assays.
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 has been used in DNA combing to determine the conversion factor.
The lambda phage has an icosahedral head and a long tail terminating in a single fiber. At both ends of the 5′ termini are complementary 12-nucleotide single strand sequences that contribute to the cohesive ends (cos region) of the DNA. The tail of the phage latches on the host outer membrane receptor and injects phage DNA into the cell. The phage converts the E. coli to a lysogenic state in which the phage functions are repressed and the phage genome may remain dormant (prophage) for a long time. This property is seen in bacteriophages that carry CII and CIII genes that are responsible for CI expression. Bacteriophages with CI mutation in the CI gene are able to maintain a lysogenic state at defined temperatures.
Infecting E. coli strain W3110 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.

包装

1 unit in glass bottle
5, 10 units in glass bottle

单位定义

One O.D. (A260) is approximately 50 μg of DNA

重悬

Add the appropriate amount of 1x Tris-EDTA buffer, pH 8.0 (Product No. T9285, diluted to 1x, or comparable), to the vial.
Incubate the DNA sample vial at 65 ℃ for 4 hrs with frequent mixing.
After reconstitution, the product should be stored at -20 ℃.

基本信息

EC 号293-505-2
MDL编号MFCD00146648
NACRESNA.52

产品性质

质量水平200
等级for molecular biology
形式lyophilized powder
分子量 31.5 × 103 kDa
48 kb
适用性suitable for substrate for restriction endonucleases
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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