产品说明

一般描述

增殖标记物 Ki-67 (MKI67) 由一个磷酸肽结合叉头相关 (FHA) 结构域和氨基端蛋白磷酸酶 1 (PP1) 结合站点、中央区 16 个串联重复序列以及羧基端亮氨酸和精氨酸 (LR) 对组成。在细胞周期的所有活动期均有表达。编码该蛋白的基因定位于人类第 10 号染色体上。
Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases (G1, S, G2, and mitosis) of the cell cycle. The gene encoding this protein is localized on human chromosome 10.

特异性

在免疫印迹中与 Ki-67 核抗原(345 和 395 kDa，以及几种较低分子量蛋白)反应。该产品显示了与其他抗 Ki-67/MIB-1 试剂相似的免疫组织化学染色模式。它在冷冻人体组织和福尔马林固定、石蜡包埋组织中标记增殖细胞。成功标记常规福尔马林固定、石蜡包埋的人体组织需要微波加热恢复步骤。

免疫原

HeLa 细胞核制备。

应用

已成功使用该抗体的应用，相关同行评议论文如下。
免疫细胞化学(1 篇论文)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry.
Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse has also been used for immunohistochemistry.

生化/生理作用

增殖标记物 Ki-67 (MKI67) 是有丝分裂染色体外周的一部分，作为生物表面活性剂在核膜解体后维持单个有丝分裂染色体分散在细胞质中。是乳腺癌和前列腺癌的预后指标。MKI67 也是一种潜在的细胞增殖标志物。
Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker. Ki-67 is considered as poor prognostic factors of adenoid cystic carcinoma.

免责声明

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