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β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

β-羟丁酸脱氢酶 来源于球形红假单胞菌

品牌
Sigma-Aldrich
CAS
9028-38-0
货号
H8509
规格纯度
Type V, lyophilized powder, 250-750 units/mg protein
参考价格
15501.3 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

包装

25 units in serum bottle

应用

适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 D(-)-3-羟基丁酸酯。

生化/生理作用

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

单位定义

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 ℃.

外形

含 Tris 缓冲盐的冻干粉

基本信息

酶学委员会(EC)编号1.1.1.30 ( BRENDA | IUBMB )
EC 号232-830-6
MDL编号MFCD00130633
NACRESNA.54

产品性质

质量水平200
类型Type V
形式lyophilized powder
specific activity250-750 units/mg protein
纯化方式chromatography
异质活性lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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