产品说明
一般描述
A cell-permeable calpain inhibitor. Inactivates calpain I (ID50 = 52 nM), calpain II (ID50 = 34 nM), and papain (ID50 = 138 nM). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca2+-ionophore induced degradation of actin binding protein and P235 in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.
A cell-permeable calpain inhibitor (ID50 = 52 nM for calpain-1; ID50 = 34 nM for calpain-2; ID50 = 138 nM for papain). Inhibits the Ca2+-stimulated cleavage of p35 to p25 by calpain. Modulates the processing of β-amyloid precursor protein to β-amyloid (Aβ). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca2+-ionophore-induced degradation of actin binding protein and P235 in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.
生化/生理作用
Cell permeable: yes
ID50 = 52 nM for calpain-1; 34 nM for calpain-2; 138 nM for papain
Product does not compete with ATP.
Reversible: no
包装
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
序列
Z-Leu-Nle-CHO
制备说明
Dissolve in a minimal amount of DMF or DMSO then add H₂O, buffer or medium just prior to use.
其他说明
Lee, M.S., et al. 2000. Nature405, 360.
Yamazaki, T., et al. 1997. Biochemistry27, 8377.
Klafki, H., et al. 1996. J. Biol. Chem.271, 28655.
Shiba, E., et al. 1996. Anticancer Res. 16, 773.
Pinter, M., et al. 1994. Neurosci. Lett. 170, 91.
Saito, K., and Nixon, R.A. 1993. Neurochem. Res. 18, 231.
Tsujinaka, T., et al. 1988. Biochem. Biophys. Res. Commun.153, 1201.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C20H30N2O4 |
分子量 | 362.46 |
MDL编号 | MFCD00155623 |
产品性质
质量水平 | 100 |
测定 | ≥94% (HPLC) |
形式 | solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
颜色 | white to off-white |
溶解性 | DMF: 5 mg/mL DMSO: 5 mg/mL |
运输 | ambient |
储存温度 | −20℃ |
InChI | 1S/C20H30N2O4/c1-4-5-11-17(13-23)21-19(24)18(12-15(2)3)22-20(25)26-14-16-9-7-6-8-10-16/h6-10,13,15,17-18H,4-5,11-12,14H2,1-3H3,(H,21,24)(H,22,25)/t17-,18-/m0/s1 |
InChI key | PGGUOGKHUUUWAF-ROUUACIJSA-N |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |