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Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor® 647 Conjugate

品牌
Sigma-Aldrich
货号
05-623-AF647
规格纯度
clone CTD4H8, from mouse, ALEXA FLUOR® 647
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

特异性

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

免疫原

Epitope: carboxyl-terminal domain of RNA polymerase II
KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

应用

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

质量

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.

目标描述

~210-220 kDa observed

外形

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
偶联物ALEXA FLUOR® 647
抗体形式purified antibody
antibody product typeprimary antibodies
克隆CTD4H8, monoclonal
species reactivityhuman, mouse
species reactivity (predicted by homology)rat (based on 100% sequence homology), yeast (based on 100% sequence homology)
technique(s)immunocytochemistry: suitable
同位素/亚型IgG1
NCBI登记号NP_000928.1
UniProt登记号P24928
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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