产品说明
一般描述
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
免疫原
Linear peptide corresponding to phospho Histone H2A.X (Ser139).
应用
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
质量
Evaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies
目标描述
~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
外形
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR® 647 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | JBW301, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | vertebrates (based on 100% sequence homology) |
| 浓度 | 0.5 mg/mL |
| technique(s) | immunocytochemistry: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NP_002096 |
| UniProt登记号 | P16104 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
