Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor^® 647

产品说明

一般描述

RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1; UniProt P31749; also known as PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID 207) in human. AKTs (protein kinase Bs) are serine/threonine kinases that play an important role in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. There exit three types of AKTs (α, β, γ or AKT 1, 2, 3) sharing a high degree of homology, but differing slightly in the localization of their regulatory phosphorylation sites. AKT α is the predominant isoform in most tissues, whereas the highest expression of AKT β is observed in the insulin-responsive tissues, and AKT γ is abundant in brain tissue. Each AKT type is composed of three functionally distinct regions, including an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

特异性

Clone SKB1 recognizes Akt1 and Akt2, but not Akt3, GST fusion proteins at 100 ng by immunoblotting analysis using the unconjugated antibody (Cat. No. 05-591).

免疫原

Epitope: Amino acids 1-149
GST-fusion protein corresponding to residues 1-149 of human Akt1.

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Akt/PKB in A431 cells.
The unconjugated antibody (Cat. No. 05-591) is shown to be suitable also for flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.
This Anti-Akt Antibody, PH Domain, clone SKB1, Alexa Fluor^® 647 is validated for use in Immunocytochemistry for the detection of PKB.
Research Category
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases

质量

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Akt/PKB in HeLa cells.

目标描述

~60 kDa observed.

外形

Protein G purified.
Purified mouse monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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