产品说明

一般描述

Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.

应用

Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).

生化/生理作用

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.
粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 是粒细胞、巨噬细胞和嗜酸性粒细胞系的生长和分化因子。GM-CSF 在极低浓度下刺激多能祖细胞集落形成，是造血祖细胞直至成熟的所有分裂中必不可少的存活和增殖因子。它也刺激一些上皮细胞和破骨细胞的生长。GM-CSF 由多种细胞类型(单核细胞、内皮细胞、T 细胞、成纤维细胞、有丝分裂原刺激的 B 细胞和 LPS 刺激的巨噬细胞)产生。GM-CSF 是一种单链糖蛋白，含有 128 个氨基酸，具有保守的二硫键。人和鼠 GM-CSF 的序列同源性约为 54%，生物活性无交叉反应。

注意

Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

外形

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

制备说明

HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

分析说明

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

法律信息

HumanKine is a registered trademark of Proteintech Group, Inc.

基本信息

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