产品介绍:
产品说明
一般描述
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
应用
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
基本信息
线性分子式 | O2NC6H4OP(O)(ONa)2 · 6H2O |
分子量 | 371.14 |
Beilstein | 5324661 |
EC 号 | 224-246-5 |
MDL编号 | MFCD00066288 |
PubChem化学物质编号 | 24897898 |
NACRES | NA.83 |
产品性质
描述 | 5 mg substrate per tablet |
质量水平 | 300 |
形式 | tablet |
荧光 | λex 310 nm (pH 7.1) λex 399 nm (Reaction Product) |
储存温度 | −20℃ |
SMILES string | O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1 |
InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 |
InChI key | KAKKHKRHCKCAGH-UHFFFAOYSA-L |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |