产品说明
应用
Interleukin-10 (IL-10) from rat has been used to study the anti-allodynic effect of IL-10 in rat model.
生化/生理作用
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
外形
Lyophilized from a 0.2 μm filtered in 20 mM Tris, 0.1 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of cytokine.
分析说明
The biological activity is measured in a cell proliferation assay using the murine mast cell line, MC/9.
基本信息
EC 号 | 201-064-4 |
MDL编号 | MFCD01635777 |
NACRES | NA.32 |
产品性质
质量水平 | 100 |
生物来源 | rat |
重组 | expressed in E. coli |
测定 | >95% (SDS-PAGE) |
形式 | lyophilized powder |
效能 | 1-35 ng/mL ED50 |
分子量 | predicted mol wt ~19 kDa |
包装 | pkg of 5 μg |
杂质 | endotoxin, tested |
UniProt登记号 | P29456 |
储存温度 | −20℃ |
Gene Information | rat ... Il10(25325) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |