产品说明
一般描述
Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor. Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.
Mouse and human Flt-3 ligand share approximately 72% homology and both demonstrate cross-species activity.
生化/生理作用
Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myeloblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP and suppresses Erk signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.
质量
Heterogenous glycosylation causes recombinant mouse Flt-3 ligand to migrate as multiple bands from 18-32 kDa.
外形
Lyophilized from a 0.2 μm filtered solution in 50% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.
分析说明
Bioactivity is measured by the ability to stimulate the proliferation of a Flt-3 transfected pro-B cell line.
基本信息
| MDL编号 | MFCD01093789 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 重组 | expressed in NSO cells |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 1.0-4.0 ng/mL |
| 分子量 | ~18 kDa |
| 包装 | pkg of 5 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P49772 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Flt3l(14256) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
