产品说明
一般描述
The expression of short hairpin RNA in eukaryotic cells. The H1 is transcribed by RNA polymerase III (RNA pol III) which terminates transcription at a string of thymidine residues typically a minimum of five residues in mammalian cells. Unlike most SnapFastTM vectors this plasmid contains an RNA pol III terminator downstream of the XhoI site although it is more common to insert a terminator sequence at the end of the sequence you insert into the MCS that encodes a shRNA.
Promoter Expression Level:
应用
Multiple cloning site notes: This vector has a modified MCS compared to standard plasmids. The ATG start codon in the NcoI site has been removed as have the Shine-Dalgarno and KOZAK sequences as these are not required for RNA Pol3 expressed transcripts.
The terminator for RNA polymerase III has been positioned immediately downstream of the XhoI site. There is an additional string of Thymidine residues (the terminator sequence for RNA Pol III) at the end of the SV40 polyA region however using this site will produce a long transcript and has not been validated. We recommend inserting your hairpin DNA sequence between Hind3 and XhoI. The theoretical RNA position +1 is the first base of the NotI restriction site.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
基本信息
| NACRES | NA.85 |
产品性质
| 形式 | buffered aqueous solution |
| 分子量 | size 3801 bp |
| 复制起点 | pUC (500 copies) |
| 肽切割 | no cleavage |
| 启动子 | Promoter name: H1 Promoter activity: siRNA Promoter type: mammalian |
| 菌种筛选 | kanamycin |
| 报告基因 | none |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
