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Avidin from egg white

亲和素 来源于鸡蛋白

品牌
Sigma-Aldrich
CAS
1405-69-2
货号
A8706
包装型号
规格纯度
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
参考价格
2649.62 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

应用

Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
亲和素蛋白用于一项分析中,该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。

包装

5 mg in glass bottle

单位定义

One unit will bind 1.0 μg of d-biotin.

制备说明

亲和纯化

基本信息

EC 号215-783-6
MDL编号MFCD00130572
NACRESNA.56

产品性质

质量水平200
重组expressed in corn
测定≥80% protein basis (biuret)
形式powder
specific activity≥12 units/mg protein (E1%/280)
分子量glycoprotein 66 kDa
subunit 16 kDa
technique(s)ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
UniProt登记号P02701
储存温度2-8℃
Gene Informationchicken ... AVD(396260)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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