产品说明
一般描述
Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges.
IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
特异性
IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997).
Possibly chicken. Occasional difficulty neutralizing rat IGF-I due to binding protein interference.
免疫原
Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986).
应用
Immunoprecipitation:
5 μg of a previous lot immunoprecipitated 100 ng of IGF-I.
Immunohistochemistry:
10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections.
(Lui, 1994).
Neutralization:
10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).
Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
质量
Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.
目标描述
17 kDa, migrates to ~7 kDa
外形
Purified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at -20ºC from date of receipt.
分析说明
Control
MCF-7 cell extracts
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
克隆 | Sm1.2, monoclonal |
species reactivity | mouse, avian, human, rat, chicken |
manufacturer/tradename | Upstate® |
technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable neutralization: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_000609 |
UniProt登记号 | P05019 |
运输 | dry ice |
Gene Information | human ... IGF1(3479) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |