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Topo I (Y723F) (mt Y723F) human

品牌
Sigma-Aldrich
货号
SRP2035
规格纯度
recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
参考价格
7018.5 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

生化/生理作用

Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Tyrosine 723 was identified as an active site for the DNA binding activity of DNA topoisomerase I. The covalent intermediate of topo I and DNA complex includes nucleophilic attack by the O4-oxygen of tyrosine 723 on a phosphester linkage in the DNA. Mutation from tyrosine to phenylalanine at position 723 preferentially binds the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNAs. But mutation at tyr723 neither affects its kinase activity that phosphorylates splicing factors of SR protein family nor its transcription activity of class II genes in vitro.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

基本信息

NACRESNA.26

产品性质

生物来源human
重组expressed in insect cells
测定≥80% (SDS-PAGE)
形式frozen liquid
分子量 ~92.4 kDa
包装pkg of 5 μg
储存条件avoid repeated freeze/thaw cycles
浓度1000 μg/mL
颜色clear colorless
NCBI登记号NM_003286
UniProt登记号P11387
运输dry ice
储存温度−70℃
Gene Informationhuman ... TOP1(7150)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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