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PSF-CMV-PURO-NH2-IL-2-INTERLEUKIN-2 SECRETION PLASMID

品牌
Sigma-Aldrich
货号
OGS1497
规格纯度
plasmid vector for molecular cloning
参考价格
5477.14 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an Human Interleukin-2 (IL-2) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

基本信息

NACRESNA.85

产品性质

形式buffered aqueous solution
分子量size 6185 bp
复制起点pUC (500 copies)
肽切割no cleavage
肽标签位置N-terminal
启动子Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
分泌信号human IL-2
菌种筛选kanamycin
哺乳动物细胞筛选puromycin
报告基因none
运输ambient
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

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