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Procathepsin L human

品牌
Sigma-Aldrich
货号
SRP6547
规格纯度
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
参考价格
3896.85 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80℃. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

外形

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in E. coli
测定≥90% (SDS-PAGE)
形式liquid
分子量 39.4 kDa (18-333 aa + N-terminal poly-his tag.)
包装pkg of 5 μg
浓度1 mg/mL
UniProt登记号P07711
运输wet ice
储存温度−70℃
Gene Informationhuman ... CTSL1(1514)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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