产品说明
生化/生理作用
MAPKAPK2 is a serine/threonine protein kinase activated by the p38 MAP kinase in response to various signals including mitogenic and stress stimuli. MAPKAPK2 is distinguished from the RSK family of ribosomal protein S6 kinases (also termed MAPKAPK1), which are activated by the MAP kinases, ERK1, and ERK2 based on its substrate specificity and amino acid sequence. Upon activation, p38 MAP kinase activates the downstream MAPKAPK2 by phosphorylation of the threonine residue in the Pro-Thr motif in response to heat shock or mitogenic stimuli. Activated MAPKAPK2 phosphorylates the small heat shock protein HSP25/HSP27, both in vitro and in vivo, at serine residues. Thus, HSP25/HSP27 seems to be the physiological substrate of MAPKAPK2.
单位定义
One unit will incorporate 1.0 nmole of phosphate into HSP 25 per minute at 30 ℃ at pH 7.4.
外形
Solution in 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.05 mM EDTA, 2 mM DTT, and 10% glycerol.
基本信息
| MDL编号 | MFCD04974881 |
| NACRES | NA.32 |
产品性质
| 重组 | expressed in HEK 293 cells |
| 质量水平 | 100 |
| 形式 | buffered aqueous glycerol solution |
| specific activity | ≥60 units/mg protein |
| 分子量 | 45-60 kDa |
| UniProt登记号 | P49137 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... MAPKAPK2(9261) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
