Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2

产品说明

一般描述

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.

特异性

Specific for the phosphorylated tyrosine residue 694 of STAT5A (94kDa) and 699 of STAT5B (92kDa). Unidentified proteins may be detected in some lysates.

免疫原

Epitope: Tyr694/699
KLH-conjugated, synthetic phosphopeptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B. Clone 8-5-2.

应用

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2 is a mouse monoclonal antibody for detection of phospho-STAT5A/B (Tyr694/699) also known as signal transducer & activator of transcription 5A/B & has been validated in WB.

质量

Routinely evaluated by Western Blot on 3T3/A31 cells stimulated with PDGF or HeLa cells stimulated with interferon-α.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected phosphorylated STAT5A/B in lysates from 3T3/NIH cells stimulated with PDGF. 0.5-2 μg/mL of a previous lot detected phosphorylated STAT5A/B in lysates from 3T3/A31 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a).

目标描述

STAT5A (94 kDa) and STAT5B (92 kDa)

外形

Purified mouse monoclonal IgG in buffer containing PBS. Frozen solution at -20°C.
Format: Purified
Protein G Purified

分析说明

Control
IFN-α induced HeLa cells or GM-CSF treated TF-1 cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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