产品说明
一般描述
A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow (λmax = 390 nm at pH 7.0) to red (λmax = 486 nm at pH 7.0) as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. Also useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. Has been used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.
包装
5 mg in Glass bottle
Packaged under inert gas
警告
Toxicity: Flammable (J)
制备说明
Preparing a Nitrocefin (500 µg/ml) Solution
• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.
• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.
• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.
• Nitrocefin, particularly in solution, is very sensitive to light.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solution may be stored at -20°C for up to 2 weeks.
其他说明
Guay, R., et al. 1980. IRCS Med. Science 8, 209.
King, A., et al. 1980. Antimicrob. Agents Chemother. 17, 165.
Matthew, M., et al. 1975. J. Gen. Microbiol. 88, 169.
O’Callaghan, C.H., et al. 1972. Antimicrob. Agents Chemother. 1, 283.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C21H16N4O8S2 |
| 分子量 | 516.50 |
产品性质
| 质量水平 | 200 |
| 测定 | ≥95% (UV) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze protect from light |
| 颜色 | orange-yellow |
| 运输 | ambient |
| 储存温度 | −20℃ |
| InChI | 1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1 |
| InChI key | LHNIIDJCEODSHA-OQRUQETBSA-N |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H317 - H318 |
| 预防措施声明 | P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
