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Superfibronectin from human plasma

品牌
Sigma-Aldrich
货号
S5171
规格纯度
solution, sterile-filtered, BioReagent, suitable for cell culture
参考价格
4197.95 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

包装

0.5 mg in poly bottle

组分

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

注意

Superfibronectin should be stored at 2-8℃ and diluted immediately prior to use. Do not freeze this product.

制备说明

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37℃ and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

基本信息

MDL编号MFCD00284706

产品性质

质量水平200
生物来源human
重组expressed in E. coli
无菌性sterile-filtered
产品线BioReagent
形式solution
分子量 ~235-270 kDa
包装pkg of 5 mg
technique(s)cell culture | mammalian: suitable
表面覆盖度 1 μg/cm2
web metadata keyword.defaultfibronectin, cell adhesion, polymeric form of fibronectin, fibronectin polymer
UniProt登记号P02751
运输ambient
储存温度2-8℃
Gene Informationhuman ... FN1(2335)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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