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SygRNA® Cas9 Synthetic Modified tracrRNA

品牌
Sigma-Aldrich
货号
TRACRRNAMOD
包装型号
规格纯度
参考价格
813.6 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The SygRNA modified synthetic RNA system recruits WT SpCas9, eSpCas9, dCas9, or Cas9D10A to specific genomic targets. 2′ O-methyl and phosphothioate modifications have been incorporated to enhance the stability and performance of the synthetic RNA. The modified tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides SpCas9 and SpCas9 variant proteins to crRNA-specified sites in the genome. SygRNA modified synthetic tracrRNA and crRNAs are optimized for use with a variety of Cas9 expressing cell lines, Cas9 proteins, or Cas9 mRNAs and are compatible with a delivery methods such as microinjection, lipofection, and electroporation.

应用

Functional Genomics/Target Validation/Transgenics

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
  • Generation of transgenic animals via embryo microinjection or electroporation

特点和优势

HPLC-purified SygRNA modified tracrRNA oligo is optimized for use with a target-specific crRNA or modified crRNA and a source of SpCas9 protein for genome editing applications.

联系

The SygRNA Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein to form active RNP complexes.
Visit SigmaAldrich.com/CRISPRessentials to to review available Cas9 sources.
Order crRNAs at SigmaAldrich.com/CRISPR

制备说明

Resuspend SygRNA modified tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

法律信息

CRISPR Use License Agreement
SygRNA is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.51

产品性质

包装pkg of 1 vial
运输ambient
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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