产品说明
一般描述
Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.
包装
5 mg in Plastic ampoule
生化/生理作用
Primary Target
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Ac-Tyr-Val-Ala-Asp-AFC
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C33H36F3N5O10 |
| 分子量 | 719.66 |
产品性质
| 质量水平 | 100 |
| 测定 | 98% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze desiccated protect from light |
| 颜色 | white to off-white |
| 溶解性 | DMSO: 5 mg/mL |
| 荧光 | λex ~400 nm λem ~505 nm |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
