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Anti-PCNA Antibody, clone 3B22 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1442
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3B22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Proliferating cell nuclear antigen (PCNA). It targets an epitope within the first 150 amino acids.

免疫原

GST/His-tagged recombinant fragment corresponding to the first 150 amino acids from the N-terminal region of human Proliferating cell nuclear antigen (PCNA).

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PCNA in HeLa cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PCNA in HeLa, A431, HUVEC cell lines.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PCNA in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant PCNA with a KD of 2.0 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PCNA, clone 3B22 ZooMAb, Cat. No. ZRB1442, is a recombinant Rabbit monoclonal antibody that specifically targets PCNA and is tested for in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

目标描述

Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (UniProt: P48454; also known as EC:3.1.3.16, CAM-PRP catalytic subunit, Calcineurin, testis-specific catalytic subunit, Calmodulin-dependent calcineurin A subunit gamma isoform, PPP3CC) is encoded by the PPP3CC (also known as CALNA3, CNA3) gene (Gene ID: 5533) in human. PPP3CC is a calcium-dependent, calmodulin stimulated protein phosphatase that plays an essential role in the transduction of intracellular Ca2+-mediated signals. It is activated by Ca2+-bound calmodulin following an increase in intracellular Ca2+. At low Ca2+ concentrations, the catalytic subunit (aa 52-343; calcineurin A) is inactive and is bound to the regulatory subunit (calcineurin B) in which only two high-affinity binding sites are occupied by Ca2+. In response to elevated calcium levels, the occupancy of the low-affinity sites on calcineurin B by Ca2+ causes a conformational change of the C-terminal regulatory domain of calcineurin A, resulting in the exposure of the calmodulin-binding domain (aa 386-400) and in the partial activation of calcineurin A. The subsequent binding of Ca2+-bound calmodulin leads to the displacement of the autoinhibitory domain (aa 451-489) from the active site and possibly of the autoinhibitory segment (aa 401-408) from the substrate binding site which fully activates calcineurin A. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3B22, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 28.77 kDa
observed mol wt 34 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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