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Anti-NOTCH2 Antibody, clone 1B14-5 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1830
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1B14-5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects NOTCH2. It targets an epitope within 18 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of human NOTCH2.

应用

Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NOTCH2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected NOTCH2 in NIH3T3 and C6 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NOTCH2 in human kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Notch2 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected NOTCH2 in MCF7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NOTCH2, clone 1B14-5 ZooMAb®, Cat. No. ZRB1830, is a recombinant Rabbit monoclonal antibody that specifically targets NOTCH2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Neurogenic locus notch homolog protein 2 (UniProt: Q04721; also known as Notch 2, hN2, NOTCH2) is encoded by the NOTCH2 gene (Gene ID: 4853) in human. Notch is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 26-2677), a transmembrane domain (aa 1678-1698), and a cytoplasmic domain (aa 1699-2471). It is evolutionarily conserved and is implicated in cell-fate determination, tissue patterning, morphogenesis, and cell differentiation. It is expressed in the brain, heart, kidney, lung, skeletal muscle and liver and is ubiquitously expressed in the embryo. Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. It serves as a receptor for Jagged-1, Jagged-2, and Delta-1 to regulate cell-fate determination. Following ligand binding, Notch is cleaved by TNF-alpha converting enzyme (S2 cleavage) to yield a membrane-associated intermediate fragment called notch extracellular truncation, which is then cleaved by g-secretase (S3 cleavage) to release a notch-derived peptide containing the intracellular domain (NICD; aa 1697-2471) from the membrane that translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yin, L., et al (2010). Biochem. Pharmacol. 80(5); 690-701).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1B14, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt ~ 265.41 kDa
observed mol wt ~ 110 kDa
species reactivityhuman, rat, mouse
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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