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Anti-NME1 Antibody, clone 1H15 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1576
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1H15 is a Rabbit recombinant monoclonal antibody that specifically detects Nucleoside diphosphate kinase A (NME1). It targets an epitope within 13 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Nucleoside diphosphate kinase A (NME1).

应用

Quality Control Testing
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NME1 in K562 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NME1 in lysates from Raji cells and Rat brain tissue.
Affinity Binding Assay: A representative lot of this antibody bound NME1 with a KD of 2.6 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NME1 in human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Nucleoside diphosphate kinase A (UniProt: P15531; also known as EC:2.7.4.6, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein) is encoded by the NME1 (also known as NDPKA, NM23) gene (Gene ID: 4830) in human. Nucleoside diphosphate kinases catalyze the reversible phosphorylation of nucleoside diphosphates (NDP) into nucleoside triphosphates (NTP) via a phosphohistidine intermediate and are responsible for cellular NDP/NTP homeostasis. Nucleoside diphosphate kinase A is present in both the cytosol and membrane fractions. It plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP g phosphate is transferred to the NDP b phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. It possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3′-5′ exonuclease activities. It is involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. It is also shown to phosphorylate ATP-citrate lyase and annexin A1. NME1 gene is considered as a metastasis suppressor gene that exhibits reduced expression in metastatic cancers and has the ability to suppress metastatic activity of cancer cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Attwood, PV., and Muimo, R. (2018). Lab. Invest. 98; 283-290; Pamidimukkala, NV., et al. (2018). Anticancer Res. 38(11); 6059-6068).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1H15, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 17.15 kDa
observed mol wt ~17 kDa
纯化方式using Protein A
species reactivityhuman, rat
species reactivity (predicted by homology)monkey
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
表位序列C-terminal
Protein ID登记号NP_000260
UniProt登记号P15531
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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