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Anti-γ-Actin Antibody, clone 6J13 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1166
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特异性

Clone 6J13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects g-Actin. It targets an epitope within 13 amino acids from the N-terminal.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human g-Actin.

应用

Anti-g-Actin, clone 6J13 ZooMAb®, Cat. No. ZRB1166, is a recombinant Rabbit monoclonal antibody that specifically targets g-Actin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected y-Actin in HepG2 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected y-Actin in HeLa, C6, and mouse kidney tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected g-Actin in human small intestine tissue sections.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected g-Actin in one million HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected g-Actin in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Actin, cytoplasmic 2 (UniProt: P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG) gene (Gene ID: 71) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. -actin is a cytoplasmic actin that is highly conserved in higher animals and is predominantly expressed in non-muscle cells where it controls cell structure and motility. Unlike β-actin that is enriched at the leading edge of migrating cells, -actin is more uniformly distributed throughout the cell. -actin knockout is reported to cause developmental delays, reduced postnatal and adult survival, and progressive myopathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Belyantseva, EA., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(24); 9703-9708).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆6J13, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt ~ 41.79 kDa
observed mol wt ~ 42 kDa
species reactivityrat, mouse, human
species reactivity (predicted by homology)bovine
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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