产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Transcriptional repressor CTCF. It targets an epitope with in 17 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 17 amino acids from C-terminal region of human Transcriptional repressor CTCF.
应用
Anti-CTCF, clone 1B18 ZooMAb, Cat. No. ZRB1009, is a recombinant Rabbit Monoclonal Antibody that specifically targets Transcriptional repressor CTCF and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CTCF in NIH3T3 and C6 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CTCF in HUVEC, HeLa, A431, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CTCF in human prostate cancer tissue sections.
目标描述
Transcriptional repressor CTCF (UniProt: P49711; also known as 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog) is encoded by the CTCF gene (Gene ID: 10664) in human. CTCF is a ubiquitously distributed chromatin binding factor that binds to DNA sequence specific sites. It contains 11 C2H2-type zinc finger regions. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF serves as a transcriptional repressor by binding to promoters of vertebrate MYC gene and BAG1 gene. It can also serve as a transcriptional activator of APP. It also plays a critical role in gene silencing over considerable distances in the genome and preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. When bound to different DNA sequences, it can dimerize to mediate long-range chromatin looping. CTCF undergoes sumolyation on lysine 74 and 689, which contributes to its repressive function on the MYC P2 promoter. Mutations in CTCF gene are known to cause mental retardation characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1B18, monoclonal recombinant monoclonal |
| 产品线 | ZooMAb® learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 82.79 kDa observed mol wt ~120 kDa |
| species reactivity | human, mouse, rat |
| 包装 | antibody small pack of 25 μL |
| 环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable using 1:100 immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100 western blot: suitable using 1:1,000 |
| 同位素/亚型 | IgG |
| 环保替代产品分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
| Gene Information | human ... CTCF(10664) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
