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Anti-Zyxin Antibody, clone 2F17 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1408
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2F17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Zyxin. It targets an epitope within 20 amino acids from the C-terminal half.

免疫原

KLH-conjugate linear peptide corresponding to 20 amino acids from the C-terminal half of human Zyxin.

应用

Anti-Zyxin, clone 2F17 ZooMAb, Cat. No. ZRB1408, is a recombinant Rabbit monoclonal antibody that targets Zyxin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zyxin in NIH/3T3 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Zyxin in human uterus and human prostate tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Zyxin in HeLa, HUVEC, and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Zyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that is widely expressed and has been implicated in cell adhesion, cell motility, and signaling. It acts as an adhesion plaque protein concentrated at sites of cell adhesion, particularly where the ends of actin filament adjoin the membrane and is thought to link the actin cytoskeletal to the membrane. It does not bind directly to actin filaments, but is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators through which it can direct the actin assembly and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. Zyxin activity is regulated by its phosphorylation. Phosphorylation of Zyxin at serine 142 is reported to increase acinus-S access to the C-terminal LIM domain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215; Chan CB., et al. (2007). Cell Death Differ. 14(9); 1688-1699; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 61.28 kDa
observed mol wt ~75 kDa
species reactivityhuman, mouse
species reactivity (predicted by homology)monkey
包装pkg of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

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