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Anti-Tyrosine Hydroxylase Antibody, clone 3M12, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB2381
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3M12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Tyrosine Hydroxylase. It targets an epitope with in 15 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat Tyrosine 3-hydroxylase.

应用

Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb, Cat. No. ZRB2381, is a recombinant Rabbit Monoclonal Antibody that specifically targets Tyrosine 3-hydroxylase and has been tested for use in Immunocytochemistry, Immunfluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Tyrosine Hydroxylase in mouse brain and rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tyrosine Hydroxylase in PC12 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Tyrosine Hydroxylase in mouse brain tissue sections.

目标描述

Tyrosine 3-monooxygenase (UniProt: P04177; also known as EC: 1.14.62.2, Tyrosine 3-hydroxylase, TH) is encoded by the TH gene (Gene ID: 25085) in rat. Tyrosine hydroxylase acts as a rate-limiting enzyme of catecholamine biosynthesis. It hydroxylates tyrosine to L-DOPA and then L- DOPA is converted to dopamine by aromatic amino acid decarboxylase. Tyrosine hydroxylase requires tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. Its activity is regulated by phosphorylation at four different serine residues by multiple protein kinases. The N-terminal region of tyrosine hydroxylase plays a critical role in controlling its catalytic activity. Its catalytic domain is located in the C-terminal region and binds the substrates and the cofactor. Its activity is inhibited by a feedback mechanism by catecholamines. Dopamine is shown to bind to tyrosine hydroxylase competitively with tetrahydrobiopterin and interacts with the regulatory domain. Tyrosine hydroxylase activity can be modified in the presence of nitric oxide that results in nitration of tyrosine residues. Tyrosine hydroxylase activity and dopamine levels are shown to be significantly lower in the brain of Parkinson s disease subjects. Tyrosine hydroxylase deficiency leads to impaired synthesis of dopamine as well as epinephrine and norepinephrine. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Daubner SC et al., (2011). Arch. Biochem. Biophys. 508(1): 1-12).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3M12, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 55.97 kDa
observed mol wt ~58 kDa
species reactivityrat, mouse
species reactivity (predicted by homology)bovine, human
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable using 1:100
immunofluorescence: 1:10-1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000
western blot: suitable using 1:1,000
同位素/亚型IgG
UniProt登记号P04177
环保替代产品分类Aligned
运输ambient
储存温度2-8℃
Gene Informationrat ... TH(25085)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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