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Anti-RAD21 Antibody, clone 2H12 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1458
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2H12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects RAD21. It targets an epitope within 102 amino acids from the C-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal half of human Double-strand-break repair protein Rad21 homolog (RAD21).

应用

Anti-RAD21, clone 2H12 ZooMAb, Cat. No. ZRB1458, is a recombinant Rabbit monoclonal antibody that specifically targets RAD21 and is tested for used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RAD21 in NIH3T3 and L6 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RAD21 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RAD21 in A431, HeLa, HUVEC, Jurkat, K562, NIH3T3, and U2OS cells.
Affinity Binding Assay: A representative lot of this antibody bound recombinant RAD21 protein fragment with a KD of 9.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Double-strand-break repair protein rad21 homolog (UniProt: O60216; also known as hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog) is encoded by the RAD21 (also known as HR21, KIAA0078, NXP1, SCC1) gene (Gene ID: 5885) in human. RAD21 is a double-strand-break repair protein rad21 homolog. It is a component of the cohesion complex and is integral to chromosome segregation, error-free DNA repair, and prevention of inappropriate recombination between repetitive regions. It is cleaved by separase/ESPL1 at the onset of anaphase and this cleavage is required for sister chromatid separation and cytokinesis. The cohesin complex also plays a role in spindle pole assembly during mitosis. RAD21 expression in colorectal cancer is associated with aggressive disease especially in KRAS mutant tumors and resistance to chemoradiotherapy. Mutations in RAD21 gene have been linked to Cornelia de Lange syndrome that is characterized by facial dysmorphisms, abnormal hands and feet, growth delay and cognitive retardation. Some mutations can also cause Mungan syndrome that leads to neuromyopathy, chronic intestinal pseudo-obstruction and a variety of cardiac valve defects. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deb, S., et al. (2014). Br. J. Cancer 110(6); 1606-1613).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2H12, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 71.69 kDa
observed mol wt 105 kDa
species reactivitymouse, human, rat
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

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