产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone LM609 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Integrin aVβ3 .

免疫原

Full length purified Integrin aV β3 from human melanoma cell line M21.

应用

Anti-Integrin aVβ3, clone LM609 ZooMAb, Cat. No. ZMS1030, is a recombinant Mouse monoclonal antibody that detects Integrin aVβ3 and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunoprecipitation.
Immunoprecipitation Analysis: A representative lot of a non-recombinant antibody immunoprecipitated Integrin Vβ3 in Immunoprecipitation applications (Jessen, T.N., et. al. (2017). Exp Cell Res. 361(2):265-276).
Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected Integrin Vβ3 in HUVEC and MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Integrin aVβ3 (UniProt: P06756/P05106; also known as CD51/CD61) is encoded by the ITGAV/ITGB3 (also known as MSK8, VNRA, VTNR/GP3A) genes (Gene ID: 3685/3690) in human. Integrin aV is a single-pass membrane protein that heterodimerizes with a beta subunit. The exact combination of various a and β subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular region of the a subunit. aV associates with either β-1, β-3, β-5, β-6, or β-8 in a non-covalent manner. The structural and functional diversity of the integrin family are based upon the pairing abilities of the individual a and β subunits. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. Integrin Vβ3 serves as a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Both Integrin aV and β3 are single-pass membrane proteins that are synthesized with a signal peptide (aa 1-30 and 1-26, respectively), which is subsequently cleaved off to generate the mature form. Their mature forms contain an extracellular domain (aa 31-992/27-718), a transmembrane domain (aa 993-1016/719-741), and a cytoplasmic domain (aa 1017-1048/742-788). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

安全信息