位置:首页 > 品牌 > Sigma-Aldrich >

Anti-cleaved PARP-1 Antibody, clone 2G13 Antibody, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1544
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2G13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects the cleaved form of poly[ADP-ribose)polymerase 1 (PARP-1). It targets an epitope within 10 amino acids from the N-terminal region consisting of the cleavage site.

免疫原

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Poly [ADP-ribose] polymerase 1 (PARP-1).

应用

Anti-cleaved PARP-1, clone 2G13, ZooMAb, Cat. No. ZRB1544, is a recombinant Rabbit monoclonal antibody that detects cleaved PARP-1 and is tested in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected cleaved PARP-1 in jurkat cells treated with etoposide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cleaved PARP-1 in HeLa cells treated with Cisplatin.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected cleaved PARP-1 in one million HeLa cell treated with Cisplatin.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected cleaved PARP-1 in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Poly [ADP-ribose] polymerase 1 (UniProt: P09874; also known as EC:2.4.2.30, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, DNA ADP-ribosyltransferase, PARP1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1) is encoded by the PARP1 (also known as ADPRT, PPOL) gene (Gene ID: 142) in human. PARP1 is zinc-dependent DNA binding protein that recognizes DNA strand breaks and plays a role in DNA repair. It is involved in the base excision repair (BER) pathway by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. In addition to BER pathway, it is also involved in double-strand breaks (DSBs) and accumulates at DNA damage sites and promotes homologous recombination repair by mediating poly-ADP-ribosylation. PARP1 also mediates the poly(ADP-ribosyl)ation of a number of proteins and mainly mediates glutamate and aspartate ADP-ribosylation of target proteins. The ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2′-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The DNA-binding region of PARP1 is localized to amino acids 2-373. It also contains two zinc-finger regions (aa 9-93 and 113-203). PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments: a 29 kDa (N-terminal) and a 85 kDa (C-terminal DNA-binding domain). The carboxyl-terminally located 54-kDa domain of PARP represents the NAD1-binding domain with the characteristic PARP signature, which includes a highly conserved sequence comprising the catalytically crucial amino acid residue Glu-988. Its catalytic activity is stimulated by noncovalent contact of the DNA-binding domain with DNA strand breaks and results in the post-translational modification of various acceptor proteins. In between the DNA binding domain and the NAD1-binding domain, it also contains a 22-kDa auto-modification domain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alvarez-Gonzalez, R., et al. (1999). J. Biol. Chem. 274(45); 32122-32126).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2G13, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 113.08 kDa (calculated for the uncleaved form. )
observed mol wt ~89 kDa ((cleaved form))
species reactivityhuman
species reactivity (predicted by homology)monkey
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号