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Anti-CD3e Antibody, clone 1H4, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1011
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1H4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects CD3e. It targets an epitope within 13 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human T-cell surface glycoprotein CD3 epsilon chain (CD3e).

应用

Flow Cytometry Analysis: 0.01 μg from a representative lot detected CD3e in Jurkat cell line.
Immunohistochemistry (Paraffin) Analysis: A 1:40 dilution from a representative lot detected CD3e in human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD3e in Jurkat cell line.
Immunofluorescence Analysis: A 1:40 dilution from a representative lot detected CD3e in human tonsil tissue sections.
Anti-CD3e, clone 1H4 ZooMAb, Cat. No. ZRB1011, is a recombinant Rabbit Monoclonal Antibody that specifically targets of CD3e T-cell surface glycoprotein and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha and beta dimer, while among 5% of T cells the TCR is composed of a gamma and a delta dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3 chain, a CD3 chain, and two CD3 chains. The CD3 complex, TCR dimer, and a -chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (aa 1-22), the removal of which yields the mature chain (aa. 23-207) with an Ig-like (aa 32-112) domain-containing extracellular region (aa 23-126), a transmembrane segment (aa 127-152), and a cytoplasmic tail (aa 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1H4, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 21.39 kDa
observed mol wt ~21 kDa
species reactivityhuman
species reactivity (predicted by homology)monkey
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)flow cytometry: 1:40-1:100
immunocytochemistry: suitable using 1:100
immunofluorescence: 1:40-1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable using 1:1,000
同位素/亚型IgG
UniProt登记号P07766
环保替代产品分类Aligned
运输ambient
储存温度2-8℃
Gene Informationhuman ... CD3E;T3E(916)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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