产品说明
一般描述
AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).
免疫原
Linear peptide corresponding to the C-terminus of mouse AIRE.
应用
Research Sub Category
Inflammation & Autoimmune Mechanisms
Western Blotting Analysis: A representative lot from an independent laboratory detected AIRE in AIRE transfected COS cells (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymus sections (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymic epithelial cells (Guerau-de-Arellano, M., et al. (2009). J Exp Med. 206(6):1245-1252.).
Detect AIRE using this rat monoclonal antibody, Anti-AIRE Antibody, clone 5H12 validated for use in Flow Cytometry, western blotting, ICC & IHC.
Research Category
Inflammation & Immunology
质量
Evaluated by Flow Cytometry in mouse primary splenocytes.
Flow Cytometry Analysis: 4 µg of this antibody detected AIRE in 1X10E6 mouse primary splenocytes.
目标描述
60 kDa calculated. Many isoforms are known to exist.
外形
Format: Purified
Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | rat |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 5H12, monoclonal |
species reactivity | mouse |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2cκ |
NCBI登记号 | NP_001258478 |
UniProt登记号 | Q9Z0E3 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |