产品说明
一般描述
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
特异性
This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.
免疫原
Fusion protein corresponding to human Nestin.
应用
Research Category
Stem Cell Research
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Anti-Nestin Antibody, clone 10C2, Alexa Fluor 488 conjugate detects level of Nestin & has been published & validated for use in ICC.
质量
Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).
目标描述
The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa
外形
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Protein A purified
储存及稳定性
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
RNcell CX neural stem cells (SCC007).
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR® 488 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 10C2, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NP_006608 |
| UniProt登记号 | P48681 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
