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Milli-Mark® Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

品牌
Sigma-Aldrich
货号
FCMAB101A4
规格纯度
clone Bu-1, Milli-Mark®, from mouse
参考价格
4848.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody′s special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.

特异性

Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.

免疫原

Bromodeoxyuridine

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by flow cytometry with Jurkat Cells.

目标描述

none given

外形

Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Protein G Purified

储存及稳定性

Stable for 6 months at 2-8°C from date of receipt. Protect from light.

分析说明

Control
After incorporation of BrdU, all DNA containing species

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
偶联物ALEXA FLUOR® 488
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆Bu-1, monoclonal
species reactivityhuman
species reactivity (predicted by homology)all
manufacturer/tradenameMilli-Mark®
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
同位素/亚型IgG2a
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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