产品说明
一般描述
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
特异性
This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
免疫原
Embryonic rat spinal cord
应用
Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.
Research Category
Stem Cell Research
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
质量
Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
目标描述
The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa
外形
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Protein G Purified
储存及稳定性
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
Mouse cortical neural stem cells (SCR029)
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR® 488 |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | rat-401, monoclonal |
| species reactivity | mouse, rat |
| should not react with | human |
| technique(s) | immunocytochemistry: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NP_037119 |
| UniProt登记号 | P21263 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
