产品说明
一般描述
This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.
Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
免疫原
Hsp90α, His•Tag
Human
包装
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)
警告
Toxicity: Irritant (B)
外形
In PBS.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
H460 and HeLa cells
其他说明
Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | EMD-17D7, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG1 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
