产品说明
一般描述
Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.
Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.
免疫原
purified mid-sized rat neurofilament (NF-M) subunit.
应用
Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- western blot analysis
- immunohistochemistry
- antibody labelling
生化/生理作用
Neurofilament 160 (NEFM) associates with the dopamine receptor.
Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01322393 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | RMO44, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rat, human, mouse, zebrafish |
| 浓度 | ~2 mg/mL |
| technique(s) | immunohistochemistry: suitable microarray: suitable western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P07197 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NEFM(4741) mouse ... Nefm(18040) rat ... Nefm(24588) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
