位置:首页 > 品牌 > Sigma-Aldrich >
产品介绍:

产品说明

一般描述

Insect GeneJuice<TMSYMBOL></TMSYMBOL> Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx<TMSYMBOL></TMSYMBOL> and pBiEx<TMSYMBOL></TMSYMBOL> vectors, which contain the immediate early baculovirus promoter, IE1.

  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 75,726 when other reagent used with Sf9, thus 1.7 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 11,952 when other reagent used with High Five, thus 11 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 31,897 when Insect Genejuice used with High Five, thus 4.1 fold difference.

Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.

包装

0.3, 1, 10 ml in Plastic ampoule

警告

Toxicity: Standard Handling (A)

法律信息

GeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
manufacturer/tradenameNovagen®
储存条件OK to freeze
technique(s)transfection: suitable
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号