产品说明
一般描述
Brain-derived neurotrophic factor (BDNF) is a protein made by neuronal cells critical for the growth and functioning of neurons as well as binding at synapses to improve signal strength between neurons. While sufficient levels of BDNF promote the function and growth of brain cells and neurons, a decrease in BDNF levels can lead to impaired neurological function and is associated with diseases such as Alzheimer′s, epilepsy, schizophrenia and depression.
The SMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
96 well assay
特异性
Cross Reactivity:
Cross reactivity and interference of ProBDNF was tested in this assay.
The assay shows 1.2% cross reactivity with ProBDNF. Serum and plasma spiked with ProBDNF show an average 4.4% interference.
Accuracy:
- Minimum of 5 EDTA plasma samples spiked with average recovery of 96% with a range of 81-108%
- Minimum of 5 serum samples spiked with average recovery of 90% with a range of 71-98%
- Minimum of 5 cerebrospinal fluid (CSF) samples spiked with average recovery of 98% with a range of 83-108%
应用
Research Category:
- Neuroscience
Research Sub-Category:
- Alzheimer′s Disease
- Neurochemistry
- Neurotrophins
- Signaling Neuroscience
The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of BDNF in human EDTA plasma, serum, and cerebrospinal fluid.
特点和优势
This new kit includes the following features:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
组分
- Assay Buffer
- BDNF Coated Beads
- Standard Diluent
- BDNF Detection Antibody
- BDNF Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
法律信息
SMC is a trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| manufacturer/tradename | SMC™ |
| assay range | sensitivity: 0.04 pg/mL (lower limit of quantification) inter-assay cv: <11% intra-assay cv: <10% ((Intra-assay CV n=20; Inter-assay n=5)) |
| technique(s) | ELISA: suitable single molecule counting: suitable |
| 相关疾病 | Alzheimer′s disease |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H302 + H332 - H311 - H319 - H373 - H412 |
| 预防措施声明 | P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P314 |
| 危险分类 | Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - STOT RE 2 Inhalation |
| 靶器官 | Respiratory Tract |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 3 |
