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pT7BlueTM-2 T-Vector-Novagen

品牌
Sigma-Aldrich
货号
69080-M
包装型号
规格纯度
参考价格
3451.92 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The pT7Blue-2 T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3′ A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue-2 vector has been specifically constructed for thisapplication and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3′ dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3′ dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA<TMSYMBOL></TMSYMBOL> Tailing Kit.
The pT7Blue-2 Vector is a versatile plasmid featuring the 43-bp Xenopus β-globin 5′-UTR for enhancement of translation in rabbit reticulocyte lysates and frog oocytes. Inserts cloned in-frame with the lacZ α-peptide coding sequence can also be expressed in E. coli from the lac promoter. The vector has many additional features that make it an excellent choice for a variety of applications.

组分

  • 2 g pT7Blue-2 T-Vector, 50 ng/l
  • 10 l Positive Control T-Vector Insert, 2.5 ng/l

警告

Toxicity: Standard Handling (A)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PT7BLUE is a trademark of EMD Chemicals Inc.

产品性质

manufacturer/tradenameNovagen®
质量水平100
储存条件OK to freeze
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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