产品介绍:
产品说明
一般描述
The pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.
包装
20 μg in Plastic ampoule
警告
Toxicity: Standard Handling (A)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
manufacturer/tradename | Novagen® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |