产品说明
一般描述
RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.
包装
1000 u in Glass bottle
警告
Toxicity: Standard Handling (A)
单位定义
One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| NACRES | NA.85 |
产品性质
| 形式 | liquid |
| manufacturer/tradename | Novagen® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
