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Anti-NeuN Antibody, clone A60, Cy3 Conjugate

品牌
Sigma-Aldrich
货号
MAB377C3
规格纯度
clone A60, from mouse, CY3 conjugate
参考价格
7197.66 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

特异性

NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.

免疫原

Purified cell nuclei from mouse brain.

应用

Detect NeuN using this Anti-NeuN Antibody, clone A60, Cy3 Conjugate validated for use in IC.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

质量

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.

目标描述

The unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa

外形

Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A purified

储存及稳定性

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
Rat E18 primary cortex neurons.

其他说明

Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Mouse, Ferret, Chicken, and Avian.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
偶联物CY3 conjugate
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆A60, monoclonal
species reactivityrat
species reactivity (predicted by homology)mouse (based on 100% sequence homology)
technique(s)immunocytochemistry: suitable
同位素/亚型IgG1
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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