产品说明
一般描述
DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.
应用
Suitable for:
- Highly specific DNA probes by nick translation
- In vitro synthesis of complementary cDNA strand
- In vitro synthesis of DNA
- Produce blunt ends from 5′ and 3′ overhangs
DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.
组分
DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.
单位定义
One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 ℃.
基本信息
| 酶学委员会(EC)编号 | 2.7.7.7 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130924 |
| NACRES | NA.53 |
产品性质
| 质量水平 | 200 |
| 等级 | for molecular biology |
| 形式 | buffered aqueous glycerol solution |
| 分子量 | 109 kDa |
| 浓度 | 5,000-15,000 units/mL |
| UniProt登记号 | P00582 |
| 异质活性 | Endonuclease, none detected |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | Escherichia coli K12 ... polA(948356) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
